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pcms egfp rtp801  (Addgene inc)


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    Addgene inc pcms egfp rtp801
    Pcms Egfp Rtp801, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcms+egfp+rtp801/pCMS-eGFP-RTP801+(Plasmid+%2365057)/bio_rxiv__2025__06__09__658667-254-20-22
    Average 88 stars, based on 8 article reviews
    pcms egfp rtp801 - by Bioz Stars, 2026-10
    88/100 stars

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    Related Articles

    Retroviral:

    Article Title: Inhibition of the STAT5/Pim Kinase Axis Enhances Cytotoxic Effects of Proteasome Inhibitors on FLT3-ITD–Positive AML Cells by Cooperatively Inhibiting the mTORC1/4EBP1/S6K/Mcl-1 Pathway
    Article Snippet: .. For construction of a retroviral expression plasmid for inducible expression of REDD1, pRevTRE-REDD1, the EcoRI/SalI fragment of pCMS-eGFP-RTP801 (Addgene, #65057) was first subcloned into the EcoRI/ XhoI site of pMXs-IG to give pMXs-IG-REDD1. .. The BamHI/SalI region of this plasmid coding REDD1 was then subcloned into the BamHI/HpaI site of pRevTRE (Clontech; Palo Alto, CA) to give pRevTRE-REDD1.

    Expressing:

    Article Title: Inhibition of the STAT5/Pim Kinase Axis Enhances Cytotoxic Effects of Proteasome Inhibitors on FLT3-ITD–Positive AML Cells by Cooperatively Inhibiting the mTORC1/4EBP1/S6K/Mcl-1 Pathway
    Article Snippet: .. For construction of a retroviral expression plasmid for inducible expression of REDD1, pRevTRE-REDD1, the EcoRI/SalI fragment of pCMS-eGFP-RTP801 (Addgene, #65057) was first subcloned into the EcoRI/ XhoI site of pMXs-IG to give pMXs-IG-REDD1. .. The BamHI/SalI region of this plasmid coding REDD1 was then subcloned into the BamHI/HpaI site of pRevTRE (Clontech; Palo Alto, CA) to give pRevTRE-REDD1.

    Article Title: ATF4 activates a transcriptional program that chronically suppresses mTOR activity promoting neurodegeneration in Parkinson’s disease models
    Article Snippet: .. Shuttle plasmids for the generation of recombinant adenoviral vectors expressing Trib3, DDIT4 and SESN2 were constructed from pcDNA-Trb3 (Addgene #131157), pCMS-eGFP- RTP801 (Addgene #65057) and pGEX-Sesn2 (Addgene #61873) respectively in a two-stage cloning process. .. In the first stage Trib3, DDIT4 and SESN2 cDNAs flanked by 5’-BamHI and 3’- EcoRI restriction sites were generated by PCR amplification (Trib3, DDIT4) or restriction enzyme digest (SESN2) of the base plasmids and inserted into the BamHI/EcoRI MCS of the pUltra-EGFP plasmid (Addgene #24129) to enable bicistronic expression of EGFP.

    Plasmid Preparation:

    Article Title: Inhibition of the STAT5/Pim Kinase Axis Enhances Cytotoxic Effects of Proteasome Inhibitors on FLT3-ITD–Positive AML Cells by Cooperatively Inhibiting the mTORC1/4EBP1/S6K/Mcl-1 Pathway
    Article Snippet: .. For construction of a retroviral expression plasmid for inducible expression of REDD1, pRevTRE-REDD1, the EcoRI/SalI fragment of pCMS-eGFP-RTP801 (Addgene, #65057) was first subcloned into the EcoRI/ XhoI site of pMXs-IG to give pMXs-IG-REDD1. .. The BamHI/SalI region of this plasmid coding REDD1 was then subcloned into the BamHI/HpaI site of pRevTRE (Clontech; Palo Alto, CA) to give pRevTRE-REDD1.

    Article Title: RTP801 is a critical factor in the neurodegeneration process of A53T α‐synuclein in a mouse model of Parkinson's disease under chronic restraint stress
    Article Snippet: RTP801 siRNA were prepared by GenePharma based on the following sequences: 5‐AAGACTCCTCATACCTGGATG‐3, which targeting both mouse and rat RTP801 sequence. .. RTP801 constructs were generated by PCR cloning (RTP801 forward, 5′‐GAATTCGAACCATGCCTAGCCTTTGGGATCG‐3′; RTP801 reverse, 5′‐CTCGAGTCAACACTCTTCAATGAGCA‐3′) from pCMS‐EGFP‐RTP801, which was a gift from Lloyd Greene & Cristina Malagelada (Addgene plasmid #65057) (Malagelada et al., 2006 ), then cut with EcoRI and XhoI enzyme sites and ligated to pcDNA3.1 vector. ..

    Construct:

    Article Title: RTP801 is a critical factor in the neurodegeneration process of A53T α‐synuclein in a mouse model of Parkinson's disease under chronic restraint stress
    Article Snippet: RTP801 siRNA were prepared by GenePharma based on the following sequences: 5‐AAGACTCCTCATACCTGGATG‐3, which targeting both mouse and rat RTP801 sequence. .. RTP801 constructs were generated by PCR cloning (RTP801 forward, 5′‐GAATTCGAACCATGCCTAGCCTTTGGGATCG‐3′; RTP801 reverse, 5′‐CTCGAGTCAACACTCTTCAATGAGCA‐3′) from pCMS‐EGFP‐RTP801, which was a gift from Lloyd Greene & Cristina Malagelada (Addgene plasmid #65057) (Malagelada et al., 2006 ), then cut with EcoRI and XhoI enzyme sites and ligated to pcDNA3.1 vector. ..

    Article Title: Loss of NEDD4 contributes to RTP801 elevation and neuron toxicity: implications for Parkinson's disease
    Article Snippet: Horseradish peroxidase-conjugated goat anti-mouse and anti-rabbit secondary antibodies were obtained from Pierce Thermo Fisher Scientific (Rockford, IL, USA). .. Goat anti-mouse and anti-rabbit secondary antibodies conjugated to Alexa 488 or Alexa 568 were purchased from Thermo Fisher Scientific (Waltham, MA, USA). pCMS-eGFP-RTP801 and pCMS-eGFP-RTP801 KR constructs were generated as previously described [ , ]. pCI-HA-NEDD4 and pcDNA3 HA-ubiquitin were purchased from Addgene (Cambridge, MA, USA). ..

    Article Title: ATF4 activates a transcriptional program that chronically suppresses mTOR activity promoting neurodegeneration in Parkinson’s disease models
    Article Snippet: .. Shuttle plasmids for the generation of recombinant adenoviral vectors expressing Trib3, DDIT4 and SESN2 were constructed from pcDNA-Trb3 (Addgene #131157), pCMS-eGFP- RTP801 (Addgene #65057) and pGEX-Sesn2 (Addgene #61873) respectively in a two-stage cloning process. .. In the first stage Trib3, DDIT4 and SESN2 cDNAs flanked by 5’-BamHI and 3’- EcoRI restriction sites were generated by PCR amplification (Trib3, DDIT4) or restriction enzyme digest (SESN2) of the base plasmids and inserted into the BamHI/EcoRI MCS of the pUltra-EGFP plasmid (Addgene #24129) to enable bicistronic expression of EGFP.

    Generated:

    Article Title: RTP801 is a critical factor in the neurodegeneration process of A53T α‐synuclein in a mouse model of Parkinson's disease under chronic restraint stress
    Article Snippet: RTP801 siRNA were prepared by GenePharma based on the following sequences: 5‐AAGACTCCTCATACCTGGATG‐3, which targeting both mouse and rat RTP801 sequence. .. RTP801 constructs were generated by PCR cloning (RTP801 forward, 5′‐GAATTCGAACCATGCCTAGCCTTTGGGATCG‐3′; RTP801 reverse, 5′‐CTCGAGTCAACACTCTTCAATGAGCA‐3′) from pCMS‐EGFP‐RTP801, which was a gift from Lloyd Greene & Cristina Malagelada (Addgene plasmid #65057) (Malagelada et al., 2006 ), then cut with EcoRI and XhoI enzyme sites and ligated to pcDNA3.1 vector. ..

    Article Title: Loss of NEDD4 contributes to RTP801 elevation and neuron toxicity: implications for Parkinson's disease
    Article Snippet: Horseradish peroxidase-conjugated goat anti-mouse and anti-rabbit secondary antibodies were obtained from Pierce Thermo Fisher Scientific (Rockford, IL, USA). .. Goat anti-mouse and anti-rabbit secondary antibodies conjugated to Alexa 488 or Alexa 568 were purchased from Thermo Fisher Scientific (Waltham, MA, USA). pCMS-eGFP-RTP801 and pCMS-eGFP-RTP801 KR constructs were generated as previously described [ , ]. pCI-HA-NEDD4 and pcDNA3 HA-ubiquitin were purchased from Addgene (Cambridge, MA, USA). ..

    Polymerase Chain Reaction:

    Article Title: RTP801 is a critical factor in the neurodegeneration process of A53T α‐synuclein in a mouse model of Parkinson's disease under chronic restraint stress
    Article Snippet: RTP801 siRNA were prepared by GenePharma based on the following sequences: 5‐AAGACTCCTCATACCTGGATG‐3, which targeting both mouse and rat RTP801 sequence. .. RTP801 constructs were generated by PCR cloning (RTP801 forward, 5′‐GAATTCGAACCATGCCTAGCCTTTGGGATCG‐3′; RTP801 reverse, 5′‐CTCGAGTCAACACTCTTCAATGAGCA‐3′) from pCMS‐EGFP‐RTP801, which was a gift from Lloyd Greene & Cristina Malagelada (Addgene plasmid #65057) (Malagelada et al., 2006 ), then cut with EcoRI and XhoI enzyme sites and ligated to pcDNA3.1 vector. ..

    Clone Assay:

    Article Title: RTP801 is a critical factor in the neurodegeneration process of A53T α‐synuclein in a mouse model of Parkinson's disease under chronic restraint stress
    Article Snippet: RTP801 siRNA were prepared by GenePharma based on the following sequences: 5‐AAGACTCCTCATACCTGGATG‐3, which targeting both mouse and rat RTP801 sequence. .. RTP801 constructs were generated by PCR cloning (RTP801 forward, 5′‐GAATTCGAACCATGCCTAGCCTTTGGGATCG‐3′; RTP801 reverse, 5′‐CTCGAGTCAACACTCTTCAATGAGCA‐3′) from pCMS‐EGFP‐RTP801, which was a gift from Lloyd Greene & Cristina Malagelada (Addgene plasmid #65057) (Malagelada et al., 2006 ), then cut with EcoRI and XhoI enzyme sites and ligated to pcDNA3.1 vector. ..

    Recombinant:

    Article Title: ATF4 activates a transcriptional program that chronically suppresses mTOR activity promoting neurodegeneration in Parkinson’s disease models
    Article Snippet: .. Shuttle plasmids for the generation of recombinant adenoviral vectors expressing Trib3, DDIT4 and SESN2 were constructed from pcDNA-Trb3 (Addgene #131157), pCMS-eGFP- RTP801 (Addgene #65057) and pGEX-Sesn2 (Addgene #61873) respectively in a two-stage cloning process. .. In the first stage Trib3, DDIT4 and SESN2 cDNAs flanked by 5’-BamHI and 3’- EcoRI restriction sites were generated by PCR amplification (Trib3, DDIT4) or restriction enzyme digest (SESN2) of the base plasmids and inserted into the BamHI/EcoRI MCS of the pUltra-EGFP plasmid (Addgene #24129) to enable bicistronic expression of EGFP.

    Cloning:

    Article Title: ATF4 activates a transcriptional program that chronically suppresses mTOR activity promoting neurodegeneration in Parkinson’s disease models
    Article Snippet: .. Shuttle plasmids for the generation of recombinant adenoviral vectors expressing Trib3, DDIT4 and SESN2 were constructed from pcDNA-Trb3 (Addgene #131157), pCMS-eGFP- RTP801 (Addgene #65057) and pGEX-Sesn2 (Addgene #61873) respectively in a two-stage cloning process. .. In the first stage Trib3, DDIT4 and SESN2 cDNAs flanked by 5’-BamHI and 3’- EcoRI restriction sites were generated by PCR amplification (Trib3, DDIT4) or restriction enzyme digest (SESN2) of the base plasmids and inserted into the BamHI/EcoRI MCS of the pUltra-EGFP plasmid (Addgene #24129) to enable bicistronic expression of EGFP.



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